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AcridineOrange
AcridineOrange

Acridine Orange

Acridine Orange (AO) is a very useful, metachromatic, vital stain. Monomers of AO intercalate into DNA specifically and fluoresce green when illuminated with blue light. Dimers and higher order aggregates of AO accumulate in RNA and fluoresce orange when illuminated with blue light. AO can also be concentrated by accumulation in acidic lysosomes (see also: QuickLeukocyteAnalysis). AO quickly passes through the plasma membrane of intact cells. Under suitable conditions AO can be used for simultaneous DnaVsRnaAnalysis.

acridine orange
excitation
emission
AO + DNA
503 nm (blue)
530 nm (green)
AO + RNA
503 nm (blue)
630 nm (red)

The 488 nm blue argon ion laser line is also quite efficient at AO excitation.


stock solution:
1 mg/ml acridine orange in water
store at 4C in the dark

typical working final concentration:
2 μg/ml in buffered solution such as PBS

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