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now: Sat Feb 24 14:25:16 2018 ... mod: Thu Jul 24 15:42:10 2014
Acridine Orange (AO) is a very useful, metachromatic, vital stain. Monomers of AO intercalate into DNA specifically and fluoresce green when illuminated with blue light. Dimers and higher order aggregates of AO accumulate in RNA and fluoresce orange when illuminated with blue light. AO can also be concentrated by accumulation in acidic lysosomes (see also: QuickLeukocyteAnalysis). AO quickly passes through the plasma membrane of intact cells. Under suitable conditions AO can be used for simultaneous DnaVsRnaAnalysis.
|acridine orange || excitation ||emission|
|AO + DNA || 503 nm (blue) ||530 nm (green)|
|AO + RNA || 503 nm (blue) ||630 nm (red)|
The 488 nm blue argon ion laser line is also quite efficient at AO excitation.
typical working final concentration:
2 μg/ml in buffered solution such as PBS