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Affinity tags for proteins
The following amino acid sequences can be fused to proteins of interest to facilitate subsequent purification. Antibodies suitable for WesternBlotting
detection of tagged proteins are available.
|Name || sequence || binds to || eluted by || comments|
|Nano-tag || DVEAWLGAR(VPLVET) || streptavidin (Kd 9mer≃17nm 15mer≃4nm) || biotin || Protein Expression and Purification 33(1)39-47(2004)|
|Strep-tag II || (SA)WSHPQFEK || strep-Tactin || desthiobiotin || http://www.iba-go.com/iba_us|
|His-tag || HHHHHH || nickel or cobalt || imidizole or low pH |
|FLAG || DYKDDDDK || antibody || EDTA or low pH || can be cleaved off by enterokinase|
|c-myc || EQKLISEEDL || antibody || low pH || usually for detection rather than purification|
|HA-tag || YPYDVPDYA || antibody || low pH || not very good|