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AffinityTag
AffinityTag

Affinity tags for proteins

The following amino acid sequences can be fused to proteins of interest to facilitate subsequent purification. Antibodies suitable for WesternBlotting detection of tagged proteins are available.

Name
sequence
binds to
eluted by
comments
Nano-tag
DVEAWLGAR(VPLVET)
streptavidin (Kd 9mer≃17nm 15mer≃4nm)
biotin
Protein Expression and Purification 33(1)39-47(2004)
Strep-tag II
(SA)WSHPQFEK
strep-Tactin
desthiobiotin
http://www.iba-go.com/iba_us
His-tag
HHHHHH
nickel or cobalt
imidizole or low pH
FLAG
DYKDDDDK
antibody
EDTA or low pH
can be cleaved off by enterokinase
c-myc
EQKLISEEDL
antibody
low pH
usually for detection rather than purification
HA-tag
YPYDVPDYA
antibody
low pH
not very good


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