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now: Thu Apr 26 16:56:13 2018 ... mod: Mon Oct 12 05:16:54 2015
Basically, chelation thrapey is used to detoxify the body and rid it of poisonous metals. It is thought to be effective for conditions and diseases such as cardiovascular disease, peripheral artery disease, coronary artery disease, carotid artery blockage, diabetes and more. It is done by using a man-made amino acid called EDTA that is infused into the veins. EDTA binds to the calcium build up on the arteries and should be removed with regular treatments. The remaining elements in the arteries would break up on their own and the nasty plaque goes away e2€“ leaving me with clean and clear arteries.
1x TAE (40 mM Tris / 40 mM Acetate / 1.0 mM EDTA)
50x TAE stock:
- 242 g Tris base
- 57.1 ml glacial acetic acid
- 100 ml of 0.5 M EDTA (pH 8.0)
bring to 1 liter total volume
store at room temperturegel running concentration is 0.5x
Although TAE doesn't have the same buffering capacity as TBE (Tris/Borate/EDTA) for almost all purposes these buffers are interchangable. TAE has the advantage of allowing a highly concentrated stock solution and lack of interference with some DNA-glass adsorption protocols.
1x TBE (89 mM Tris / 89 mM Borate/ 2.0 mM EDTA)
5x TBE stock, 1 liter final volume:
- 53.9 g Tris base
- 27.5 g Boric acid
- 20 ml of a 500 mM EDTA solution, pH 8.0
- do NOT just use EDTA powder. the pH 8.0 EDTA solution contributes significant ionic strength from added NaOH
gel running concentration is 0.5x
5x stock TBE is fairly reliable. 10x TBE tends to have solubility problems