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1x TAE (40 mM Tris / 40 mM Acetate / 1.0 mM EDTA)

50x TAE stock:

bring to 1 liter total volume
store at room temperture
gel running concentration is 0.5x

Although TAE doesn't have the same buffering capacity as TBE (Tris/Borate/EDTA) for almost all purposes these buffers are interchangable. TAE has the advantage of allowing a highly concentrated stock solution and lack of interference with some DNA-glass adsorption protocols.

1x TBE (89 mM Tris / 89 mM Borate/ 2.0 mM EDTA)

5x TBE stock, 1 liter final volume:

gel running concentration is 0.5x

5x stock TBE is fairly reliable. 10x TBE tends to have solubility problems

Basically, chelation thrapey is used to detoxify the body and rid it of poisonous metals. It is thought to be effective for conditions and diseases such as cardiovascular disease, peripheral artery disease, coronary artery disease, carotid artery blockage, diabetes and more. It is done by using a man-made amino acid called EDTA that is infused into the veins. EDTA binds to the calcium build up on the arteries and should be removed with regular treatments. The remaining elements in the arteries would break up on their own and the nasty plaque goes away e2€“ leaving me with clean and clear arteries.
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