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BacterialAntibiotic
BacterialAntibiotic

Antibiotics commonly used with bacterial plasmids for growth in E. Coli

Antibiotic
Stock Concentration
Storage
Working Concentration
Dilution
ampicillin
50 mg/ml in water
-20C
100 μg/ml
1:500
chloramphenicol
34 mg/ml in ethanol
-20C
170 μg/ml
1:200
kanamycin
10 mg/ml in water
-20C
50 μg/ml
1:200
streptomycin
10 mg/ml in water
-20C
50 μg/ml
1:200
tetracycline
5 mg/ml in ethanol
-20C
50 μg/ml
1:100
carbenicillin
50 mg/ml in water
-20C
50 μg/ml
1:1000
zeocin
100 mg/ml in water
-20C
25 μg/ml
1:4000

Be sure that media (eg LuriaBroth) has cooled to 50C or less before adding antibiotics. At this temperature, the container should feel very hot, but should be able to be held in bare hands.


Low Copy Plasmids

Most modern plasmids have been heavily modified to achieve very high copy number in bacterial cells. Older plasmids (based on naturally occurring plasmid sequences) are low in copy number, usually around 5 copies per cell. If using a low copy number plasmid, reduce the amount of antibiotics added

Antibiotic
Working Concentration
ampilcillin
20 μg/ml
chloramphenicol
25 μg/ml
kanamycin
10 μg/ml
streptomycin
10 μg/ml
tetracycline
10 μg/ml
carbenicillin
20 μg/ml

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