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now: Wed Nov 21 03:04:06 2018 ... mod: Mon Oct 12 04:54:03 2015
I totally unrntsdaed! I'm dreading Y and seriously considering going with the one Y I managed to scrape up while searching for previous letters. Z isn't going to be much fun either. UNLESS I could grab my camera and run to the parks with a day of Y and Z hunting. THAT would be awesome.
Colonies that express functional LacZ will appear blue.
- 0.55 g X-gal
- 0.06 g IPTG
- add dimethylformamide to 10 ml
- note that dimethylformamide will very rapidly degrade polyethylene and polycarbonate ("clear" plastics). use glass and/or polypropylene
store at -20C
Spread 100 μl per 10 cm agar bacterial plate for visualization of LacZ alpha-complementation.
alternatively, buy it premade from Sigma for extra money
- It is often helpful to place plates at 4C for several hours after colonies have grown to enhance visualization of the blue color.