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Flow Cytometry

Weakly phosphate buffered (pH 7.4) saline solution
(per L)
0.5x Isotonic
145.4 mM → 8.5g
72.7 mM → 4.25g
4.86 mM → 0.69g anhydrous
2.50 mM → 0.36g anhydrous
1.25 mM → 0.15g anhydrous
0.75 mM → 0.09g anhydrous
acridine orange
to 1 μg/ml final concentration
to 4 μg/ml final concentration

Sheath fluid: 1x PBS with ProClin 300 (500 μl per liter) added as an anti-bacterial / anti-fungal.

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