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PhosphateBufferedSaline
PhosphateBufferedSaline

Phosphate Buffered Saline (PBS)

PBS is the "water" of the cell culture world. It is mostly isotonic and buffered for pH. Cells aren't too happy if left in PBS for extended lengths of time, but are usually ok with short exposures. PBS is good for rinsing cells and is also a standard sheath fluid for FlowCytometry.

1x PBS
per 1 L
per 4 L
[final]
NaCl
8.0 g
32 g
137 mM
KCl
0.2 g
0.8 g
2.7 mM
Na2HPO4 (anhydrous)
1.44 g
5.76 g
10 mM
KH2PO4 (anhydrous)
0.24 g
0.96 g
1.8 mM

final pH after dilution to 1 liter should be somewhere between 7.4 and 7.6 (can be adjusted with HCl if desired).

m1 * v1 = m2 * v2You haven't stated how much you want to make, which is the v2 paameetrr. Lets assume you want 2mL of this diluted solution.m1=300mMv1=unknownm2=7.5mMv2=2mLv1=m2 * v2 / m1v1 = 0.05 mLFor the solute you want a 5% = 1:20 ratio of DMSO:PBSSo use 50uL of the original solution plus 50uL of DMSO plus 1.9mL of PBS.
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