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now: Wed Oct 18 21:29:00 2017 ... mod: Mon Jul 1 23:28:17 2013
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StreakForIsolation
StreakForIsolation

Streak for Isolation

From a culture of mixed or concentrated bacteria, dilute the sample by streaking on an agar plate so that colonies arising from individual cells can be isolated.

  1. sterilize a bacterial loop by flaming
  2. allow the loop to cool slightly and gather a small sample of either bacterial culture or a small scraping of a frozen bacterial stock onto the loop
    • for frozen stocks, do not allow the stock to thaw
  3. streak the loop across the top of a bacterial agar plate in three parallel lines
    • the lines should be fairly close together
    • streak using the wide part of the loop, not the edge of the loop
  4. flame sterilize the loop and cool by stabbing a clean part of the agar plate
  5. make three new parallel streaks starting by going across the last part of the previous three streaks and continuing onto a clean part of the plate
    • repeat the last two steps three or four times being careful to only contact the immediately previous streaks and not earlier streaks
  6. grow at 37C overnight


Comments

Great insight! That's the answer we've been lkonoig for.
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