Affinity tags for proteins
The following amino acid sequences can be fused to proteins of interest to facilitate subsequent purification. Antibodies suitable for
WesternBlotting detection of tagged proteins are available.
Name
| sequence
| binds to
| eluted by
| comments |
Nano-tag
| DVEAWLGAR(VPLVET)
| streptavidin (Kd 9mer≃17nm 15mer≃4nm)
| biotin
| Protein Expression and Purification 33(1)39-47(2004) |
Strep-tag II
| (SA)WSHPQFEK
| strep-Tactin
| desthiobiotin
| http://www.iba-go.com/iba_us |
His-tag
| HHHHHH
| nickel or cobalt
| imidizole or low pH
| |
FLAG
| DYKDDDDK
| antibody
| EDTA or low pH
| can be cleaved off by enterokinase |
c-myc
| EQKLISEEDL
| antibody
| low pH
| usually for detection rather than purification |
HA-tag
| YPYDVPDYA
| antibody
| low pH
| not very good |
Comments