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now: Thu Jun 24 09:03:51 2021 ... mod: Tue Mar 30 16:03:51 2021
50x TAE stock:
Although TAE doesn't have the same buffering capacity as TBE (Tris/Borate/EDTA) for almost all purposes these buffers are interchangable. TAE has the advantage of allowing a highly concentrated stock solution and lack of interference with some DNA-glass adsorption protocols.
1x TBE (89 mM Tris / 89 mM Borate/ 2.0 mM EDTA)
5x TBE stock, 1 liter final volume:
gel running concentration is 0.5x
5x stock TBE is fairly reliable. 10x TBE tends to have solubility problems