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AgaroseGelBuffer
AgaroseGelBuffer
1x TAE (40 mM Tris / 40 mM Acetate / 1.0 mM EDTA)

50x TAE stock:

bring to 1 liter total volume
store at room temperture
gel running concentration is 0.5x

Although TAE doesn't have the same buffering capacity as TBE (Tris/Borate/EDTA) for almost all purposes these buffers are interchangable. TAE has the advantage of allowing a highly concentrated stock solution and lack of interference with some DNA-glass adsorption protocols.


1x TBE (89 mM Tris / 89 mM Borate/ 2.0 mM EDTA)

5x TBE stock, 1 liter final volume:

gel running concentration is 0.5x

5x stock TBE is fairly reliable. 10x TBE tends to have solubility problems

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