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now: Fri Sep 17 05:34:47 2021 ... mod: Sun Apr 4 18:44:29 2021
Colonies that express functional LacZ will appear blue.
- 0.55 g X-gal
- 0.06 g IPTG
- add dimethylformamide to 10 ml
- note that dimethylformamide will very rapidly degrade polyethylene and polycarbonate ("clear" plastics). use glass and/or polypropylene
store at -20C
Spread 100 μl per 10 cm agar bacterial plate for visualization of LacZ alpha-complementation.
alternatively, buy it premade from Sigma for extra money
- It is often helpful to place plates at 4C for several hours after colonies have grown to enhance visualization of the blue color.