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Weakly phosphate buffered (pH 7.4) saline solution
(per L) | Isotonic | 0.5x Isotonic |
NaCl | 145.4 mM → 8.5g | 72.7 mM → 4.25g |
Na2HPO4 | 4.86 mM → 0.69g anhydrous | 2.50 mM → 0.36g anhydrous |
NaH2PO4 | 1.25 mM → 0.15g anhydrous | 0.75 mM → 0.09g anhydrous |
acridine orange | to 1 μg/ml final concentration | to 4 μg/ml final concentration |
Sheath fluid: 1x PBS with ProClin 300 (500 μl per liter) added as an anti-bacterial / anti-fungal.