FlowCytometry

Flow Cytometry

Weakly phosphate buffered (pH 7.4) saline solution
(per L)
Isotonic
0.5x Isotonic

NaCl
145.4 mM → 8.5g
72.7 mM → 4.25g

Na₂HPO₄
4.86 mM → 0.69g anhydrous
2.50 mM → 0.36g anhydrous

NaH₂PO₄
1.25 mM → 0.15g anhydrous
0.75 mM → 0.09g anhydrous

acridine orange
to 1 μg/ml final concentration
to 4 μg/ml final concentration

Sheath fluid: 1x PBS with ProClin 300 (500 μl per liter) added as an anti-bacterial / anti-fungal.

(per L)	Isotonic	0.5x Isotonic
NaCl	145.4 mM → 8.5g	72.7 mM → 4.25g
Na₂HPO₄	4.86 mM → 0.69g anhydrous	2.50 mM → 0.36g anhydrous
NaH₂PO₄	1.25 mM → 0.15g anhydrous	0.75 mM → 0.09g anhydrous
acridine orange	to 1 μg/ml final concentration	to 4 μg/ml final concentration