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now: Thu Jun 24 09:08:24 2021 ... mod: Sat Mar 27 08:11:34 2021
can be frozen for indefinite long-term storage at -80C in the presence of 15% glycerol.
- StreakForIsolation and grow a small overnight culture from a single colony
- add 300 μl of sterile 50% glycerol to 700 μl of the overnight culture
- mix well by pipetting up and down and store at -80C
- Filter sterilize the 50% glycerol (by volume with water) solution and store at room temperature.