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now: Mon May 29 12:22:23 2023 ... mod: Sat Mar 27 08:11:34 2021
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Glycerol Stock

E. coli can be frozen for indefinite long-term storage at -80C in the presence of 15% glycerol.

  1. StreakForIsolation and grow a small overnight culture from a single colony
  2. add 300 μl of sterile 50% glycerol to 700 μl of the overnight culture
  3. mix well by pipetting up and down and store at -80C


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