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MiniProteanThreeRecipe
MiniProteanThreeRecipe

Recipes for the Bio-Rad Mini Protean III SDS-PAGE system

See also the long MiniProteanThreeInstructions without recipes.

The percentage of the resolving gel determines the molecular weights of proteins which can be resolved. 12% is a good generic gel to use and will resolve proteins from 15 kDa to 200 kDa with emphasis on resolving proteins from 15 kDa to 100 kDa. Lower percentage resolving gels will do a better job of resolving larger proteins, but will allow smaller molecular weight (MW) species to migrate off the gel. Higher percentage gels will similarly give extra resolution to lower MW species at a sacrifice of higher MW resolution.

resolving gel
6%
8%
10%
12%
40% acrylamide (29:1 crosslink)
600 μl
800 μl
1.0 ml
1.2 ml
4x resolving buffer pH 8.8
1 ml
1 ml
1 ml
1 ml
H2O
2.4 ml
2.2 ml
2.0 ml
1.8 ml
10% APS
20 μl
20 μl
20 μl
20 μl
TEMED
2 μl
2 μl
2 μl
2 μl
Total volume
4.0 ml
4.0 ml
4.0 ml
4.0 ml

stacking gel
one gel
two gels
40% acrylamide (29:1 crosslink)
117 μl
234 μl
4x stacking buffer pH 6.8
300 μl
600 μl
H2O
783 μl
1566 μl
10% APS
12 μl
24 μl
TEMED
2 μl
4 μl
Total volume
1200 μl
2400 μl

The Mini Protean III needs about 350 ml of running buffer:

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