6x Native Loading Dye
mix:
- 600 μl 50% glycerol in H2O
- 50 μl 1% bromophenol blue dissolved in ethanol
- 50 μl 1% xylene cyanol dissolved in ethanol
- 60 μl 1 M tris pH 8.0
- 60 μl 500 mM EDTA
- 60 μl 10% SDS
- 120 μl H2O
add 1 μl of loading dye per 5 μl aqueous solution